|
KMID : 1004820110120040195
|
|
Journal of Biomedical Research
2011 Volume.12 No. 4 p.195 ~ p.203
|
|
|
Effects of Angiotensin II on Podocyte Synaptopodin: Role of AMP Kinase
|
|
Ha Tae-Sun
Park Hye-Young
Choi Ji-Young
Nam Ja-Ae
Ahn Hee-Yul
|
|
|
Abstract
|
|
|
|
As a sensor of cellular energy status, AMP-activated protein kinase (AMPK) plays an important role in the pathophysiology of diabetes and its complications. Because AMPK is also expressed in podocytes, podocyte AMPK would be an important factor contributing to development of podocyte injury. We investigated the roles of AMPK in the pathological changes of podocyte synaptopodin induced by angiotensin II (Ang II), a major injury inducer. Mouse podocytes were incubated in media containing various concentrations of Ang II and AMPK-modulating agents, and the changes of synaptopodin were analyzed by confocal imaging and Western blotting. Ang II and compound C, an AMPK inhibitor, concentrated and re-localized synaptopodin from peripheral cytoplasm to the internal cytoplasm portion in podocytes. Ang II also reduced synaptopodin protein and mRNA, which were reversed by metformin and 5-aminoimidazole-4-carboxamide ribonucleoside. Losartan, an Ang II type 1 receptor antagonist, also recovered synaptopodin mRNA, which was suppressed by Ang II. We suggest that Ang II induces the relocation and suppression of podocyte synaptopodin by suppression of AMPK and via Ang II type 1 receptor, which would be an important mechanism in Ang II-induced podocyte phenotypical changes.
|
|
|
KEYWORD
|
|
|
AMP kinase, Angiotensin II, Podocyte, Synaptopodin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|